Research scholarly Contribution:
Numerous publications to highly impacted and IF journals, e.g. Journal of Functional Foods (IF=4.48), J. Agric Food Chem. (IF=3.10), Molecular Nutrition & Food Research (IF=4.7), Analytical Chemistry (IF= 5.87), Biochimica et Biophysica Acta (BBA) (IF=5.1), Journal of Nutrition (4.70), Allergy (IF=6.8) etc
- Peptide-based immunotherapy of egg allergy (supported from NSERC Discovery Grant).
Peptide-based immunotherapy (PIT) represents an attractive approach for targeted interventions in immunological disorders, but has not been widely explored in the context of food allergy. We have successfully induced tolerance in egg allergic mouse via PIT. The mechanistic pathways entailing pivotal molecules TGF- and FOXp3 were highlighted as promising trails for the understanding of immunotherapeutic approaches for food allergy.
- Oral immunotherapy with immunodominant T-cell epitope peptides alleviates allergic reactions in a Balb/c mouse model of egg allergy (NSERC Discovery grant).
Allergen-specific T cell epitopes are obvious targets for immunotherapeutic interventions in allergic disease. T cell epitope peptides given orally may provide a practical way of inducing tolerance and preventing allergy. We investigated oral immunotherapy (OIT) with T-cell epitope peptides of the dominant egg-white allergen Ovomucoid (Ovm) in a Balb/c mouse model of egg allergy and demonstrated that OIT with peptides comprising the immunodominant regions of Ovm was safe and significantly reduced subsequent frequency of allergy to Ovm and validates potential use of Ovm T-cell epitope as an immunoregulator.
- Role of the extracellular calcium-sensing receptor in chronic inflammation (supported from AFMNet discovery grant).
The extracellular calcium-sensing receptor (CaSR) is the first identified G protein-coupled receptor. Previously, g-glutamyl cysteine and -glutamyl valine(?-EC and ?-EV) were identified as novel positive allosteric modulator of CaSR through the allosteric binding. We also demonstrated that activation of CaSR by g-EC or gŠEV could suppress TNF-?-induced inflammatory responses in intestinal epithelial cells (IECs), and reduced intestinal inflammation in a mouse model of colitis. The CaSR-mediated anti-inflammatory effects of the peptides were abrogated in ?-arrestin2 knockout IECs, and involvement of ?-arrestin2 was found to inhibit the TNF-?-dependent pro-inflammatory signaling cascade via cross-talk with the TNF-? receptor (TNFR). Further detailed analysis using gene ontology (GO) analysis clarified the role of CaSR plays as a therapeutic target for chronic inflammation, Our research strongly suggests the CaSR must play a central role in many complex disease conditions such as hypertension, cancer, diabetes, atherosclerosis and kidney disorders.
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- Novel anti-oxidative stress egg yolk phosphopeptides (supported from AFMNet, Discovery grant and Star Program, OMAF MRI and ISOP).
We along with our industrial partners are in the process of developing health promoting egg yolk peptides with anti-oxidative stress activity. We have found egg yolk phosvitin-derived phosphopeptides reduce pro-inflammatory interleukin-8 secretion, lower lipid peroxidation byproducts and increase superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR), and the glutathione (GSH) precursor gamma-glutamyl cysteine synthetase (?-GCS) in vitro and in vivo models. In this study, we identified least two consecutive phosphoserine residues (PP-2) are necessary for the modulation of cellular oxidative stress. The effect of PP-2 on the gene expression profile was measured with PCR-array analysis and gene ontology (GO) analysis was used to determine the biological roles and molecular functions. In conclusion, PP-2 can reduce intestinal oxidative stress via the activation of Nrf2-ARE (nuclear factor- E2-related factor 2 Š antioxidant response element) signalling pathway. We have demonstrated that egg yolk peptides (EYP) with anti oxidative stress properties were capable of improving gut health and mitigating the onset of the chronic disease. We have successfully developed an industry-friendly method to prepare and characterize peptides for this purpose prior to use in animal trials.
- Non-nutritional biological activities of amino acids to improve chronic gut inflammation (supported from AFMNet Discovery grant).
We have discovered that L-tryptophan and L-cysteine exhibit therapeutic functions in a porcine model of dextran sodium sulfate (DSS)-induced colitis. The L-cysteine and L-tryptophan were both examined for ability to reduce colitis symptoms and modulate local gene expression and we found that both amino acids reduced inflammation and enhanced the rate of recovery of DSS-induced colitis in pigs. Our outcomes offer a novel therapeutic approach for the treatment of chronic inflammatory disorders via dietary amino acid supplements.
- Impact of soy derived di-/tri peptides in chronic inflammation (supported from OMAFRA/UG Food Program).
We recently demonstrated that a soy-derived hydrolysate, enriched in di- and tripeptides, exerted anti-inflammatory activity in vivo in a porcine model of dextran sodium sulfate (DSS)-induced inflammation. The orally administered soy peptides prevented DSS-induced changes in gut permeability and histology, and reduced myeloperoxidase (MPO) activity and the expression of inflammatory cytokines tumor necrosis factor (TNF)-?, interleukin (IL)-6, interferon (IFN)-?, IL-1?, and IL-17A in the colon. VPY was identified as a novel PepT1 substrate that can inhibit the production of pro-inflammatory mediators in vitro in intestinal epithelial and immune cells, and reduce the severity of colitis in mice by down-regulating the expression of pro-inflammatory cytokines in the colon, suggesting that VPY may be promising for the treatment of IBD.
- Journal Associate editor
Associate Editor for J. Agric. Food Chem. (American Chemical Society), a top food science journal since 2010 and first Associate Editor from Canadian Institute.
- Editorial Board
Int. J. Food Sci. and Tech. (UK) (2004-) Editorial Board Member.
J. Functional Foods (USA) (2008-) Editorial Board Member
Food Bioscience (UK) (2012-) Editorial Board Member
International Advisory Committee
International Nutraceutical and Functional Food Society
International Symposium on Egg Science & Technology
International Conference on Food Science and Technology
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